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Raman, B., Bluemke, D
A study conducted at the University of North Carolina found that methionine solutions stored at 25C for 48 hours retained only 68% of their initial methyl donor capacity when tested in hepatocyte cell cultures
Dried peptides were resuspended in 0.1% TFA and Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS) performed in single replicate injections as previously described 93 using an Ultimate 3000 nano-HPLC coupled to a Q-Exactive mass spectrometer (Thermo Scientific) via an EASY-Spray source
A slow, steady injection over 5 to 10 seconds is preferable
