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3.5 WNK1-TRPC6-NFATc1 pathway WNK1 in ccRCC cells regulates Ca 2+ signal by activating Gq-coupled receptor/PLC- pathway, further mediates the activation of transient receptor potential cation channel 6 (TRPC6) and up-regulates the level of NFATc1

Different reconstitution volumes produce different concentrations from the same vial: 50mg vial + 2ml water = 25mg/ml (very concentrated) 50mg vial + 2.5ml water = 20mg/ml (concentrated) 50mg vial + 5ml water = 10mg/ml (standard) 50mg vial + 10ml water = 5mg/ml (dilute) Higher concentrations require smaller injection volumes but may exceed solubility limits for some peptides

2.5 mg dose in a 12 mg vial: Target 50 units (0.5 mL)

A., Flory, J., Rowan, C

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