Immunoblot analysis of protein expression Cells were lysed with 0.1% Triton X-100 in TBS (10 mM TrisHCl and 150 mM NaCl, pH 7.4) in the presence of protease inhibitors (complete, 04693132001, Roche)
In particular, the expression of RUNX2, OSX and OCN was remarkably increased at day 3, and the expression of COL-I was raised at day 7, while the expression of DMP1 was upregulated at day 3 and 7 (Fig
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