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The SELS rs34713741 polymorphism is associated with susceptibility to colorectal cancer and gastric cancer: a meta-analysis

That rules out, straightforwardly, radioligand or fluorescent binding assays, competition binding against a known ligand, recombinant receptor-expressing lines, functional readouts tied to a defined coupling pathway, and antagonist controls to demonstrate that an effect is receptor-mediated

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The next step is arguably one of the most critical, yet it's where we see so many well-intentioned researchers stumble: reconstitution