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3a and 3c)

ROS formation was quantified from a DCF-standard curve and data are expressed as pmol DCF formed/min/mg protein

Moreover, IR has been associated with microalbuminuria even in normoglycemia, and thus can be present regardless of diabetes diagnosis

Sigma, Saint Louis, Missouri) Oxidative stress was assessed by the quantification of protein carbonyls and MDA using Protein Carbonyl Colorimetric and TBARS Assay Kits, respectively (Cayman Chemical, Ann Arbor, MI)