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Following withdrawal of NFT, Nrf2 levels rapidly declined below the detection limit, whereas GCLC levels remained significantly higher than those observed in the untreated control even after 24 h in the absence of NFT

6.1 Glutathione metabolism: modern view of biosynthetic pathways, NADPH dependence, and relationship to thioredoxin The synthesis of glutathione (GSH) predominantly occurs through the ATP-dependent -glutamyl cycle, beginning with the action of glutamate-cysteine ligase (GCL), the key rate-limiting enzyme composed of two subunits: a catalytic component (GCLC) and a regulatory modifier (GCLM)

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