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Initial optimization (months 1-3)

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After 1.5 h incubation at room temperature (RT), the membrane was solubilized in the buffer above supplemented with 0.5% (w/v) lauryl maltose neopentyl glycol (LMNG, Anatrace) and 0.1% (w/v) cholesterol hemisuccinate (CHS, Anatrace) for 2 h at 4C

it appears to recapitulate the intracellular signals that exercise generates, including AMPK activation, mitochondrial biogenesis, and GLUT4 upregulation, in tissues that can no longer experience them through movement

However, it can occur indirectly due to side effects like nausea, bloating, or increased anxiety, especially when adjusting to the medication