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The lysates were centrifuged (10.000 g , 30 min, 4 C) and the supernatants were loaded on equilibrated Ni-NTA agarose columns (Qiagen)

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Out of those participants, 85% lost at least 5% of their weight (versus 26% with placebo), 69% lost at least 10% (vs 12%), 54% lost at least 15% (vs 6%), and 34% lost at least 20% (vs 3%)