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The EMPA-REG OUTCOME trial showed that empagliflozin markedly reduced the risk of CV mortality compared to that observed with placebo [8]

Flow cytometry was performed using a BD Fortessa instrument (BD) or NovoCyte Quanteon and data were analyzed using FlowJo v10.6.1 software (Tree Star)

The supernatant was then removed and resultant formazan crystals were solubilized in 150 l DMSO

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