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Further, these spectra displayed a strong band in a spectral range of 500530 cm 1 that was assigned to two partially overlapping bands: The (S-S) of GSSG centred at 508 cm 1 and (S-N) of GSNO at 522 cm 1

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MS1 and MS2 accuracy, and retention time window scans, were set to 0 allowing DIA-NN to perform their automatic inference using first run in the experiment (DIA-NN recommended MS1 mass accuracy setting: 12.7639 ppm)