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The full-length PGRMC1 cDNA fragment containing resistant sequences for shRNA was generated by using the primers (Supplementary Methods), and ligated into the Eco RI and Bam HI sites of the C-terminus of the 3xFLAG-tagged expression vector p3xFLAG CMV14 (Sigma)

Aged garlic extract was also found to suppress in vitro angiogenesis by inhibiting endothelial cell proliferation, loss of adhesion, motility, and tube formation (78)

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Yu, W., He, X., Vanommeslaeghe, K