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Mol Metab (2018) 8:11731

Following post-fixation, the skin was embedded in artificial medium (Shandon Cryomatrix, 120 mL, Thermo Scientific, USA), frozen, and cut into 20-m sections on a cryostat (Thermo, Microm International FSE, Germany)

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Reduced GSH levels shown by decreases in this ratioboth in the plasma and intracellularlyreflect intracellular shifts in oxidative burden and are indicative of immune dysfunction and progression of various disease states such as infection, sepsis, and age-related immune system decline (Guerra et al., 2011

& Schultz, M