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Briefly, cells were fixed, processed, mounted on Epon stubs, polymerized at 60 C, and 70 nm sections cut en face with a diamond knife (DiATOME) in a Leica Ultracut UCT ultramicrotome before examination on an FEI Tecnai G2-Spirit TEM

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Thus, cystine limitation suppressed mTORC1 and activated the ISR, the two major amino acid sensing pathways in HepG2 cells

10.1007/s00774-024-01554-y 29 LeeH