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Methylfolate is required in the activation of tetrahydrobiopterin (i.e., BH4), which is a necessary cofactor for both tryptophan hydroxylase and tyrosine hydroxylase, enzymes required in the synthesis of serotonin and norepinephrine/dopamine, respectively (Figure 1)
Pyridoxal phosphate (PLP) is integral to one-carbon metabolism, a series of biochemical reactions necessary for the synthesis of nucleotides and other molecules essential for DNA and RNA production (18, 57, 58)
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