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( d ) Brain-wide Fos expression over area across three groups of mice: WT:Gnas fl/fl [saline] (n = 4 mice), WT:Gnas fl/fl [sema] (n = 7 mice), Glp1r-ires-cre:Gnas fl/fl [sema] (n = 5 mice)
Seino (Kobe University, Japan), were used between passages 15 and 25 and grown in DMEM containing 10% (vol/vol) fetal calf serum, 10 mM HEPES, 2 mM L-glutamine, 1 mM sodium pyruvate, and 0.05 mM 2-mercaptoethanol at 37 C with 5% CO 2
Courtesy of Dr Carl Allen, Ohio State University
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