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Yes, I did increase the frequency of injections, up to every other day because of the neurological symptoms, and whilst this has effected minor improvements, the major symptoms are still present (muscle stiffness, pain, weakness, etc.)
In brief, a solution of calcein (Sigma, USA) was administered intraperitoneally in phosphate buffered saline (PBS) at a dosage of 10 mg/kg on the 1st and 8th days prior before sacrifice
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10.1016/j.plaphy.2010.08.016 45 GomathiR.RakkiyapanP
Henna has a fine texture that dissolves quickly
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