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Tissues were manually homogenized using a glass-glass potter in a pH 7.4 cold buffer solution containing 150 mM NaCl, 50 mM Tris HCl, and 5 mM EDTA in the presence of a complete set of proteases (Roche, Monza, Italy), and phosphatase inhibitors (Merck Life Science S.r.l, Milano, Italy)

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A researcher intending 200mcg who draws 0.2ml from a 1mg/ml solution administers 200mcg correctly

The essential amino acids (EAA) content was generally low for all the protein isolates (Table 3)