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Then cells were resuspended in tag-mentation buffer (10 mM MgCl2 in Dig-med Buffer) and incubated at 37 C for 1 h
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A second portion of the endometrial biopsy was placed in RNAlater (Sigma-Aldrich Canada, Oakville, ON, Canada), kept at 4 C overnight, and then stored at 80 C until processed for nucleic acid extraction
4.3 Oxygen transport agents Agents like trans-sodium crocetinate (TSC) (C 20 H 22 Na 2 O 4 ), derived from crocetin (C 20 H 24 O 4 ), interact with water molecules to form a densely packed matrix that enhances oxygen diffusion into hypoxic tissue sites (156)
however, if levels become too high there can be damage to cell's DNA and protein
