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Full names for all the enzymes and substrates are given in Additional File 1

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GLP-1 Daily Support Fiber is a low-FODMAP blend of soluble and insoluble fibers with added postbiotic strains for enhanced SCFA production, long-term metabolic support, and digestive regularity.* Each of the ingredients in GLP-1 Daily Support Fiber was selected to help maintain a healthy gastrointestinal environment by supporting the gut microbiome and easing common digestive side effects Resistant potato starch (Solnul) This prebiotic promotes the growth of Akkermansia muciniphila, keystone gut bacteria essential for boosting GLP-1 signaling and enhancing gut barrier function.* A 50:50 soluble-insoluble fiber blend supports digestive health and regularity while improving long-term metabolic function.* This specific prebiotic inhibits DPP-IV enzyme activity, extending the lifespan of GLP-1 for prolonged effects on glucose regulation and hunger suppression.* Partially hydrolyzed guar gum (Sunfiber) This highly fermentable fiber and producer of butyrate supports digestive regularity and satiety.*L

Epidemiological data from the International Diabetes Federation (IDF) indicate that the global burden of diabetes affected approximately 463 million adults in 2019, with projections estimating a dramatic increase to 700 million cases by 2045 2

Contaminants or fillers can reduce efficacy and pose health risks