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separation occurs in the terminal ileum in the presence of calcium, and vitamin B 12enters the mucosal cell for absorption

On the day of assay 2 g per well of the cell membrane was incubated with furimazine (1:1000 dilution from stock) in assay buffer (1 HBSS, 10 mM HEPES, 0.1% (w/v) BSA, 1 P8340 protease inhibitor cocktail, 1 mM DTT and 0.1 mM PMSF, pH 7.4)

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