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In protocols exploring caloric restriction mimetics or NAD+ restoration therapies, 5-Amino-1MQ serves as a pharmacological tool to replicate the metabolic effects of energy deficit without actual caloric restriction

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Intrinsic factor, a protein that shuttles B12 through the absorption process, becomes saturated after a meal containing an above-average amount of B12 and about 4 to 6 hours are required before it can efficiently absorb another dose (IOM, 1998)