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cDNA was prepared from total RNA and used for gene expression by real-time quantitative RTPCR (qPCR) as described [53] using TaqMan Gene Expression Assays (ThermoFischer Scientific) specific for human GCLC (Hs00155249_m1), OCT4 (Hs00999632_g1), and ACTB (Hs01060665_g1)
Because MICT consistently elevates circulating BDNF, it represents a rational foundational prescription when neuroprotection is a primary therapeutic goal (217, 232)
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Instead of starting high, we titrate up gradually and monitor closely
Springer, New York, NY, pp 167180 Floh L, Toppo S, Orian L (2022) The glutathione peroxidase family: discoveries and mechanism
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