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The -cyano amide-based Michael acceptors have also been elegantly used by the Taunton group as warheads in small-molecule inhibitors to react with active site cysteines while being minimally affected by the high concentration of intracellular GSH due to the fast reaction kinetics and the mM of K d (refs 31, 32)

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Throughout the study, safety assessments included physical examinations, vital sign measurements, electrocardiograms, laboratory testing, adverse event monitoring, and the administration of the Beck Scale for Suicidal Ideation

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