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Before imaging, the growth medium was removed, then the cells were washed with PBS (2) and imaged in FluoroBrite DMEM
Proper positioning of the feet helps prevent pressure on the heels, reducing the risk of skin breakdown and pressure ulcers in patients who are seated for extended periods
Routes of Drug Administration Chapter 6 Giovannitti JA, Trapp LD (1991) Adult sedation: oral, rectal, IM, IV
Cell culture Human malignant melanoma (A375) cells were obtained from the American Type Culture Collection (ATCC, CRL-1619IG-2) and used within ten passages for all experiments
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