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Acetyl-CoA carboxylase (ACC) inhibition demonstrates therapeutic potential for metabolic syndrome by modulating lipid biosynthesis and energy homeostasis (Harwood, 2005)
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On the day of the assay, serial dilutions (7-fold dilutions, 7 concentrations per compound and one well containing only assay buffer) of test compounds were performed in duplicate in assay buffer to appropriate concentrations in 96-well plates (Greiner, U-shape #GR-650201) using a Biomek i7 liquid handler
Poon HF, Calabrese V, Calvani M, Butterfield DA
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