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An aliquot from the wells containing the most active LacAnc100 and LaccID clones was inoculated in 3 ml of YPD (10 g l 1 yeast extract (Gibco, 212750), 20 g l 1 peptone (Gibco, 211677), 20 g l 1 d-glucose (Thermo Scientific, 410955000) and 25 g ml 1 chloramphenicol (Merck, C0378)) and incubated at 30 C and 225 rpm for 24 h