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To 1 ml of OD 600 40 culture, 10 l probe ( Metro-P1 , Metro-P2 or Metro-P3 , 100 stock in DMSO, final concentration 1 M) or 1% (v/v) DMSO were added and incubated at 37 C (140 rpm, microaerophilic conditions) for 2 h and subsequently collected by centrifugation (6,000 g , 10 min, 4 C)

Methods 20 , 11791182 (2023)

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In contrast, a microdose might start at just 0.05 mg to 0.25 mg weeklyoften less than one-tenth the full dose

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