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In: Proceedings of international conference on life Science and Technology

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Because embryonic stem (ES) cells give rise to all the tissues in an organism, mechanisms are in place to avoid the accumulation of DNA to prevent mutations that would transmit to tissues and subsequent generations

Single colonies were picked, expanded, and dissociated into clumps using 1 mg/mL collagenase type IV (Gibco, Thermo Fisher Scientific, #17104019)