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Despite the moderate sample size, the pooling strategy effectively reduced within-group variation, revealing statistically significant differences in multiple key quality parameters such as sperm motility and antioxidant enzyme activities [46]
Our roGFP2 measurements confirm (i) that dynamic roGFP2 sensing (or the physiological glutathionylation/deglutathionylation of regular surface-exposed cysteinyl residues) requires a glutaredoxin activity, and (ii) that glutathionylated proteins or metabolites should be very transient redox species when GSH and glutaredoxins are present (unless they are stabilized by a kinetic uncoupling mechanism) 5,32,34,35,61,62,63,64
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Zaugg et al., 2020)
Mingorance C et al
