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Peptide hormones work by binding to specific receptors on the surface of target cells, initiating a cascade of intracellular events that result in the desired biological effect
In brief, HUVECs were lysed in the Co-IP buffer containing 20 mM Tris-HCl (pH7.5), 100 mM NaCl, 0.5 mM EDTA, 0.5% NP-40, 0.5 mM PMSF, 0.5% protease inhibitor cocktail on ice for 20 min
Pediatr Nephrol 23(12):21592173
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