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Applications in Research As a molecular probe for studying melanocortin receptor signalling Investigation of GPCR-mediated intracellular signalling pathways In vitro studies examining receptor selectivity and peptide stability Use as a reference compound in neuroendocrine and peptide signalling research Specifications Summary Purity (HPLC): 99 % Appearance: White to off-white lyophilised powder Molecular Weight: ~1025.18 g/mol Peptide Type: Synthetic melanocortin analogue Solubility: Water, buffered aqueous solutions Storage: 20 C, desiccated, protected from light Pack Size: 10 mg Handling, Reconstitution & Stability Weigh under dry conditions
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[0081] 42.6 g of crude Semaglutide was weighed, dissolved in 1500 mL of a mixed solvent of 25% acetonitrile and 75% water, and purified using Waters 2545 RP-HPLC system with a wavelength of 218 nm, a chromatographic column of 50 x 250 mm reverse phase C18 column, a column temperature of 45C, and a conventional 0.1% TFA/acetonitrile mobile phase
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