Samples were fixed in 10% buffered formalin overnight at 4C, dehydrated with increasing concentrations of ethanol, embedded in paraffin, cut into 5 m sections, and stained with hematoxylin and eosin (HE) or Massons Trichrome Stain Kit (Sigma-Aldrich)
Ethics declarations All procedures complied with the standards for care and use of animal subjects as stated in the Guide for the Care and Use of Laboratory Animals (Institute of Laboratory Resources, National Academy of Sciences, Bethesda, MD)
NNMT has been described as a regulator of cellular energy metabolism, redox balance, and methylation-dependent signaling networks
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People with chronic kidney disease, due to reduced kidney functions and reduced excretion 8