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Introduction of shRNA to knock down ACTL6A expression inhibited the wound healing and metastatic ability of GC cells (Fig
Glutathione depletion as a consequence occurs in: active chronic infection (Epstein-Barr reactivation, chronic hepatitis B/C, HIV, tuberculosis, Lyme co-infections), uncontrolled diabetes with HbA1c above 8.5%, ongoing heavy metal exposure (occupational, dental amalgam in symptomatic individuals, contaminated water), persistent mold biotoxin exposure, untreated obstructive sleep apnea with nocturnal hypoxia, and chronic NSAID, acetaminophen, or alcohol use
Crucially, in all three cell lines, the de novo GSH synthesis inhibitor buthionine sulfoximine (BSO) abolished the ability of MPA and Pyr pretreatment to inhibit ferroptosis, consistent with a GSH-dependent protective mechanism (Fig 4B)
The Antioxidant Tetrapeptide Epitalon Enhances Delayed Wound Healing in an in Vitro Model