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The automated microscopy approach we used to demonstrate this has certain advantages over lower throughput methods, by allowing responses to a wide range of ligands or, in this case, ligand concentrations to be monitored in parallel across several FOVs, characterising ligand effects in more detail and with greater statistical robustness

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[12] The glutathione molecule binds in a cleft between N - and C -terminal domains - the catalytically important residues are proposed to reside in the N -terminal domain