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The most common substitution is oxidized GSH (GSSG), or GSH blended with masking agents, both detectable by HPLC but not by appearance alone

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Repeated freeze-thaw cycles can degrade the peptide structure, much like vigorous shaking can

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Second, the TTH sample ( n = 19) may have been underpowered to detect subtle GSH alterations, and the conclusion that GSH reduction is specific to migraine should be interpreted cautiously pending replication with larger TTH cohorts that account for potential heterogeneity (e.g., episodic frequency, coexisting conditions)