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At the cellular level, B12 acts as a cofactor for two critical enzymatic reactions: Methionine synthase converts homocysteine to methionine, essential for DNA methylation and synthesis Methylmalonyl-CoA mutase involved in fatty acid metabolism and myelin production By restoring adequate B12 levels, injections enable normal red blood cell maturation in the bone marrow, correcting the megaloblastic anaemia

Unlike standard sterile water for research which contains no preservative and must be used immediately after opening, bacteriostatic water maintains sterility through multiple punctures, making it ideal for laboratory applications requiring frequent use or multi-application protocols

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To visualize the cellular uptake hECs were cultured in 96-well black plates with clear bottom (7 10 3 cells/well