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Plasma which was not deproteinized prior to storage was removed from storage (80 o C and 4 o C, respectively) and mixed with an equal volume of cold 5% SSA and incubated for 10 min at 4 o C (on ice)
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The mass spectrometer parameters collected data from 80 to 500 m/z at an acquisition rate of 17 spectra/sec 39
Limited range of motion
The mixture was centrifuged at 4C and 12,000 g in a refrigerated centrifuge and the supernatant was collected
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