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W.AmanteJ

Vitamin-C-rich supplements reduce blood uric acid levels and lower the risk of gout- a type of arthritis

The qRT-PCR primer sets for cDNA amplification were as follows: TNF- (forward: 5-TCCAGGCGGTGCCTATGT-3/reverse: 5-CACCCCGAAGTTCAGTAGACAGA-3), IL-6 (forward: 5-CTGCAAGAGACTTCCATCCAGTT-3/reverse: 5-AAGTAGGG AAGGCCGTGGTT-3), IFN- (forward: 5-TCAAGTGGCATAGATGTGGAAGAA-3/reverse: 5-TGGCTCTGCAGGATTTTCATG-3), CXCL2 (forward: 5-CTCAGTGC TGCACTGGTCCTG-3/reverse: 5-CTGGGGGCGTCACACTCAAG C-3), and GAPDH (forward: 5-CCTCGTCCCGTAGACAAAATG-3/reverse: 5-TCTCCACTTT GCCACTGCAA-3)

Importantly, as shown in the first row, LASSS supplementation effectively prevented an increase in the nitration level of ECM-HGF, whereas GSSSG did not demonstrate efficacy, inconsistent with its potent potential shown in in vitro experiments described earlier (see Figure 1b)

At first glance, it may seem like higher oral doses would work better, but evidence suggests that is not always true