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A Multicenter, Randomized, Double Blind, Placebo Controlled Phase II Study of PL 14736 Enema in the Treatment of Mild-To-Moderate Ulcerative Colitis

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The volume of saline added the dilution directly affects how the toxin behaves: Concentrated reconstitution (lower saline volume) smaller injection volume, less diffusion, more precise effect, but requires more accurate placement Dilute reconstitution (higher saline volume) larger injection volume, more diffusion, easier to inject but more risk of toxin spreading to unintended muscles We use a standard reconstitution of 100 units in 2.5ml of unpreserved saline a precise concentration that gives accurate dosing without excessive diffusion

If the same vial were diluted with 4mL of water, the concentration would be halved to 2,500mcg/mL, and the same 2,500mcg dose would now require 1mL, or 100 units