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First , for the purposes of determining whether the concentration of a Section 4 Substance is high enough to amount to an anti-doping rule violation, Anti-Doping Organisations ( ADOs ) rely on the concentration estimates reported by the relevant WADA-accredited laboratories, rather than a Validated Quantification Procedure as used to assess the concentration of Threshold Substances

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Sections were incubated in a cocktail containing probes (Probe dilution: 50C1:1C2:1C3) for 2 h at 40C in a HybEZTM oven (ACD)

These agents target multiple metabolic pathways involved in appetite regulation, insulin secretion, glucose homeostasis, and energy expenditure, making them particularly relevant for adolescents with obesity and associated metabolic complications