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This supports the hypothesis about the hemolysis-preventing role of eryptosis, since detrimental internal or external perturbations triggering eryptosis might continue to act, overcoming the adaptive capacity of cells, causing lysis
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Real-time quantitative PCR Total ribonucleic acid (RNA) was extracted from murine chondrocytes or macrophages using the ReliaPrep RNA Cell Miniprep System (Promega, Charbonnire-les-Bains, France)