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Moreover, biochemical analyses were performed on whole brain homogenates, assessing malondialdehyde, glutathione and catalase as oxidative stress markers using standard ELISA and spectrophotometric assays

Use any remaining solution in the old vial for your next injection as a "top-up" if needed, though most researchers simply discard the last remnant rather than risk imprecise dosing

[12] The glutathione molecule binds in a cleft between N - and C -terminal domains - the catalytically important residues are proposed to reside in the N -terminal domain