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Steady state is reached in 4 to 5 weeks of weekly dosing at each dose level -- which is why the standard titration holds each dose for 4 weeks before escalating

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Mouse models of MTHFR deficiency The Mthfr of mice were knockout to investigate MTHFR deficient by animal models 38
General timing principles Separate by at least 1 hour

A systematic review on NAC supplementation for neuroinflammation and neurodegenerative diseases found that there are promising studies demonstrating the potential of NAC, but more studies are necessary due to the differences in the studies
