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This sgRNA effectively introduced a stop codon at the glutamine residue corresponding to amino acid 116 of TRIM37 (termed Trim37 ko3 ) by intracytoplasmic co-injection with the hA3A-eBE3-Y130F base editor, 33 leading to complete PGC deficiency at E12.5 (33 homozygotes out of 36 embryos) (Supplementary information, Fig

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