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Protein extract (40 l) was added to 175 l of K-phosphate buffer pH 7.5 and 25 l of 5 mM GSSG (Sigma G-6654)

This results in sustained elevation of GH and IGF-1 levels

The concentration of extracted RNA was determined with spectrophotometer (Pico drop Real-Life) and total RNA suspended in 10l of DEPC water was stored at 80C.19 Following extraction of all RNA samples, DNase I (Roche, Basel, Switzerland) was used to remove contamination induced by genomic DNA

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