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Confocal microscopy analysis of fixed human colon carcinoma BE cells in telophase showed that depletion of RhoA by siRNA (siRhoA) or treatment of cells with Y16, an inhibitor of G-protein-coupled RhoA GEFs (RhoAi), diminished actin patches compared with untransfected or vehicle (DMSO) controls, and this was not due to chromatin bridge breakage or to reduced total levels of actin by western blotting (Fig

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