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After this, samples were reduced with 10 mM dithiothreitol and alkylated with 55 mM iodoacetamide

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These profiles indicated that the TMB electro-oxidation catalyzed by pFeSAN and FeSAN proceeded via a two successive one-electron oxidation: (1) to yield an intermediate product TMB-free radical and then (2) to give the completely oxidized product quinonediimine

For testing, animals were anesthetized with ketamine (80 mg/kg) and xylazine (10 mg/kg) via i.p