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Moreover, expression of a selenium-independent GPX4 mutant enables cells to survive cystine-free media.Our model is that the function of exogenous cystine in cell culture media is principally to enable selenium import and selenoprotein function, particularly that of GPX4, and that this function can be substituted by beta-mercaptoethanol.Overall, we conclude that in vitro culture conditions substantially contribute to an overestimation of our ability to induce ferroptosis in tumors

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& Wieringa, B

T.GaberyS.MarstalK.AlanentaloT.MercerA