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Membranes were pretreated with 5% non-fat milk/TBST (1 h) before overnight exposure at 4 C to antibodies targeting MAPKAPK2 (ab32567), HO-1 (ab189491), KEAP1 (ab119403), Nrf2 (ab137550), -H2AX (ab81299), PARP-1 (ab191217), CHK2 (ab207446), phosphorylated CHK2 (p-CHK2, ab59408), TYR (ab170905), PMEL (ab137078), MITF (ab12039), p-HSP27 (ab155987), HSP27 (ab12351), -actin (ab8226), and Histone H3 (ab1791) (all from Abcam, UK)
Vitamin C, vitamin E, CoQ10, resveratrol, glutathione, ergothioneine, and astaxanthin each play different roles in supporting the bodys antioxidant defense system
Bioconjug Chem
As we age or face heightened environmental exposure (think Torontos winter air pollution and harsh weather), glutathione levels naturally decrease, leading to dullness, uneven skin tone, and more pronounced signs of aging
