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Glutaredoxins employ parallel monothiol-dithiol mechanisms to catalyze thiol-disulfide exchanges with protein disulfides

, Fas ligand, cortisol) but also by intrinsic mitochondrial pathways ( e.g

While the peak for GSH (2.98 min) gradually decreased, the signal corresponding to GSSG increased (4.37 min), indicative of the expected chemical transformation (Fig